Izitshalo zokwelapha zendabuko ziye zaziswa eminyakeni edlule ngokunikeza ukuqonda ngezinhlobonhlobo zezifo.Nokho, ukuhlukanisa ama-molecule athile asebenzayo endaweni yezinhlanganisela ezakha izinhlobo eziningi zezitshalo kungaba umsebenzi onzima.Manje, abacwaningi eNyuvesi yaseToyama, eJapane baye benza indlela yokuhlukanisa nokuhlonza izinhlanganisela ezisebenzayo emithini yezitshalo.
Idatha entsha—eshicilelwe kamuva nje ku-Frontiers in Pharmacology esihlokweni esithi, “Isu Elihlelekile Lokuthola Umuthi Wokwelapha we-Alzheimer's Disease kanye ne-Target Molecule“, bonisa ukuthi indlela entsha ikhomba izinhlanganisela eziningana ezisebenzayo ezivela ku-Drynaria rhizome, umuthi wezitshalo wendabuko, othuthukisa inkumbulo futhi wehlise izici zesifo kumodeli yegundane lesifo i-Alzheimer's.
Ngokuvamile, ososayensi bazohlola ngokuphindaphindiwe imithi yezitshalo ezingahluziwe ekuhlolweni kwelebhu ukuze babone ukuthi ingabe ikhona yini inhlanganisela ebonisa umthelela kumaseli atshalwe ku-vitro.Uma inhlanganisela ibonisa umphumela omuhle kumaseli noma amashubhu okuhlola, ingase isetshenziswe njengesidakamizwa, futhi ososayensi bayaqhubeka nokuyihlola ezilwaneni.Nokho, le nqubo iwumsebenzi onzima futhi ayibaleli izinguquko ezingaba khona ezidakamizweni lapho zingena emzimbeni—ama-enzyme asegazini nesibindi angagaya izidakamizwa zibe izinhlobo ezihlukahlukene ezibizwa ngokuthi ama-metabolite.Ukwengeza, ezinye izindawo zomzimba, njengobuchopho, kunzima ukufinyelela kuzo izidakamizwa eziningi, futhi izidakamizwa ezithile kuphela noma ama-metabolites azo azongena kulezi zicubu.
"Izithako ezikhonjwe ezikrinini zemithi yezitshalo ezisezingeni eliphezulu azihlali zisebenza ngempela ngoba lezi zivivinyo azinaki i-biometabolism kanye nokusatshalaliswa kwezicubu," kuchaza umphenyi wocwaningo ophezulu u-Chihiro Tohda, Ph.D., uprofesa ohlanganyele we-neuropharmacology eNyuvesi yaseToyama. ."Ngakho-ke, sihlose ukuthuthukisa izindlela ezisebenza kahle kakhulu zokuhlonza izinhlanganisela ezisebenzayo ezicabangela lezi zici."
Ocwaningweni, ithimba le-Toyama lasebenzisa amagundane anokuguqulwa kofuzo njengemodeli yesifo i-Alzheimer's.Lokhu kuguquka kunikeza amagundane izici ezithile zesifo i-Alzheimer's, okuhlanganisa inkumbulo encishisiwe kanye nokunqwabelana kwamaprotheni athile ebuchosheni, abizwa ngama-amyloid kanye nama-tau amaprotheni.
"Sibika isu elihlelekile lokuhlola abantu abasebenzisa i-bioactive emithini yemvelo esetshenziselwa isifo i-Alzheimer's (AD)," kubhala ababhali.“Sithole ukuthi i-Drynaria rhizome ingathuthukisa ukusebenza kwenkumbulo futhi ithuthukise i-AD pathologies kumagundane ayi-5XFAD.Ukuhlaziywa kwamakhemikhali ezinto eziphilayo kwaholela ekuhlonzweni kwama-metabolite asebenzayo adluliselwa ebuchosheni, okuyi-naringenin nama-glucuronides ayo.Ukuze sihlole indlela yokwenza, sihlanganise ukuzinza kwethagethi esabelayo ehambisana nezidakamizwa nokuhlaziywa kwe-immunoprecipitation-liquid chromatography/mass spectrometry, sihlonza iphrotheni yokusabela kwe-collapsin ye-protein 2 (CRMP2) njengento ehlosiwe ye-naringenin.”
Ososayensi bathola ukuthi ukukhishwa kwesitshalo kunciphisa ukukhubazeka kwenkumbulo kanye namazinga we-amyloid ne-tau amaprotheni ebuchosheni begundane.Ngaphezu kwalokho, ithimba libe selihlola izicubu zobuchopho begundane ngemva kwamahora amahlanu lilapha amagundane nge-extract.Bathola ukuthi izinhlanganisela ezintathu zesitshalo zazingene ebuchosheni—i-naringenin nama-naringenin metabolites amabili.
Lapho abaphenyi bephatha amagundane nge-naringenin emsulwa, baqaphela ukuthuthuka okufanayo kokusilela kwenkumbulo kanye nokuncipha kwamaprotheni e-amyloid kanye ne-tau, okusho ukuthi i-naringenin nama-metabolite ayo cishe kwakuyizinhlanganisela ezisebenzayo ngaphakathi kwesitshalo.Bathole iphrotheni ebizwa ngokuthi i-CRMP2 i-naringenin ebophezela kuyo kuma-neurons, okubangela ukuthi akhule, okuphakamisa ukuthi lena kungaba indlela i-naringenin engathuthukisa ngayo izimpawu zesifo i-Alzheimer's.
Abacwaningi banethemba lokuthi indlela entsha ingasetshenziswa ukukhomba ezinye izindlela zokwelapha.“Sisebenzisa le ndlela ukuze sithole imithi emisha yezinye izifo ezinjengokulimala komgogodla, ukucindezeleka, ne-sarcopenia,” kuphawula uDkt. Tohda.
Isikhathi sokuthumela: Mar-23-2022